Mycoplasma Detection
Introduction Mycoplasma Detection is the process of determining whether a product or article is contaminated with, or has been produced by, mycoplasma. Mycoplasma are the smallest known motile organisms and can cause serious economic losses for the livestock industry. Detection Methods 1. Physical Examination Idiopathic lesions are identified in animals that may indicate infection with mycoplasmas. Lesions suggestive of mycoplasmal disease include mouth abscesses, sores in the nose, patches on the skin (e.g. scabs or scurf), blisters and ulcers on the skin, pyoderma, and otitis media. Common identification methods include fecal samples, throat and nasal swabs, trachea, conjunctiva, and tissue samples (e.g. lung tissue). 2. Culture of Mycoplasma An important aspect of mycoplasma detection is isolating the bacterium. Mycoplasmas are fastidious organisms that require specific culture media to grow properly. Multiple media formulations and conditions (e.g. heat treatment